Abstract:We will develop a novel system for directed nucleic acid cleavage. This system is based uponour discovery that the 5' nucleases of several thermostable DNA polymerases can be directed to cleaveas site-specific endonucleases. This system will be thermostable, specific, efficient and easilyautomatable… more
Abstract:Surgeons performing arthroplasty on painful, misshapen, arthritic hand joints do not have the choice of replacing the damaged joint with a prosthesis that will restore near natural movement as they would if a hip joint were being replaced. There are no effective finger joint replacements available.… more
Abstract:The presence of high concentrations of organic solvents constitutes an extreme environment for microorganisms, yet little work has been done to isolate or develop microorganisms suitable for use under such conditions. There is a need for solvent-resistant microorganisms for use in a variety of such… more
Abstract:We will develop a biosensor system for rapid detection of specific toxic metals and organic compounds in blood or urine. Chemical-specific biosensors are produced by fusing chemical-responsive regulatory genes and reporter lux genes. By transforming an appropriate host with the resulting fusion… more
Abstract:THE OVERALL OBJECTIVE OF THIS RESEARCH IS TO DEVELOP A HIGHLY AUTOMATED DNA SEQUENCING SYSTEM THAT CAN BE SOLD TO MOLECULAR BIOLOGY RESEARCHERS INTERESTED IN CARRYING OUT LARGE SCALE SEQUENCING PROJECTS. THE COMPLETE SYSTEM WILL INCLUDE ROBOTIC REACTION PREPARATION, HIGH CAPACITY GEL ELECTROPHORESIS… more
Abstract:AN INDIRECT MARKER-TRAIT RELATIONSHIP OCCURS WHEN THE GENETIC MARKER IS LOCATED NEAR THE CONTROLLING GENE. BECAUSE THE MARK AND TRAIT ARE LINKED THROUGH ASSOCIATION, THE MARKER-TRAIT RELATIONSHIP BETWEEN TWO INDIVIDUALS MAY NOT BE THE SAME. A GENETIC MARKER HAS BEEN USED TO DETECT A SIGNIFICANCE… more
Abstract:We will develop a dual-section quadrature detection (QD) volume foot and ankle coil which can either be interfaced with a magnetic resonance imaging (MRI) system as a switched field-of-view (FOV) or a multiple channel coil to facilitate imaging both anatomical regions without patient re-positioning.… more
Abstract:A PROTOTYPE FOR AN EXPLOSIVE DETECTION SYSTEM USING PULSED FAST NEUTRON ACTIVATION (PFNA) TECHNIQUES USES AN ELECTROSTATIC TANDEM ACCELERATOR TO PRODUCE NANOSECOND WIDTH PULSES OF 6 MEV DEUTERONS. WHILE THIS APPROACH ALLOWS ONE TO PRODUCE NEUTRONS OF AN ENERGY SUITED TO THE REACTION ONE HOPES TO… more
Abstract:THE STORAGE OF LIQUID CRYOGENS IN ZERO-G CONDITIONS WITH MINIMAL CRYOGEN LOSS AND HIGH DELIVERY RATES HAS BEEN RECOGNIZED AS AN ESSENTIAL REQUIREMENT IN SPACE APPLICATIONS FOR MANY YEARS. THE SUGGESTION IN THIS INVITATION TO CONSIDER A STORAGE SYSYEM THAT UTILIZES THE MAGNETIC PROPERTIES OF THE… more
Abstract:The northward migration from Brazil of the Africanized honey bee (AHB), Apis mellifera scutellata, following their accidental release presents a new U. S. public health concern. Because of the AHB's extremely defensive colony behavior, persons may be attacked by swarms; this will result in hundreds… more
Abstract:ORBITEC proposes to develop a means to produce and utilize cyrogenic solid-based hydrogen fuels in a combustion chamber. Controlled freezing from the gaseous phase at or below the triple point assures uniform solid phase formation directly from gaseous phase. The vapor pressure of hydrogen is… more
Abstract:A RESEARCH AND EVALUATION PROJECT TO DETERMINE THE FEASIBILITY AND FORM OF A FULLY AUTOMATED NATIONWIDE DIRECT DIAL TELEPHONE NUMBER DIRECTORY ASSISTANCE SERVICE FOR THE DEAF CONTAINING TELEPHONE NUMBERS OF ALL INDIVIDUALS AND BUSINESSES OWNING TDD/TTYS, WHICH WILL BE ACCESSIBLE BY TDDS… more
Abstract:THIS PROJECT DEALS WITH THE CREATION OF "EARTHLAB", A MODEL-BASED VISUALLY RICH INTERACTIVE LEARNING ENVIRONMENT FOR EARTH SCIENCE EDUCATION. IT IS BUILT ON A SYSTEM ARCHITECTURE DESIGNED TO SUPPORT INTERNETWORKING THAT WOULD FACILITATE AN INTEGRATED CURRICULUM, PROBLEM SOLVING, AND… more
Abstract:MICROTUBER USAGE HAS BEEN LIMITED BY HIGH LABOR INPUTS NEEDED FOR PRODUCTION. USING THE PATENTED MICROTUBER MULTIPLICATION SYSTEMS (MMS), THE MULTIPLICATION FACTOR IS INCREASED OVER 25 FOLD. THE INCREASE IN MULTIPLICATION FACTOR SIGNIFICANTLY REDUCES MICROTUBER PRODUCTION COSTS. IN THIS PROPOSAL… more
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