A model will be developed to create effective vaccine vectors that will elicit mucosal (secretory) immunity. recombinant vaccinia virus that express one or more antigensof the pseudorabies virus (prv) will be used as the prototype in the design of the first-generation vectors. the ld50 (the dose… More

In order to create a vaccine strain attenuated for virulencethree strains of vaccinia virus the new york city board of health vaccine strain and two more virulent derivatives, thewestern reserve and the international health department j strains, will be used to prepare recombinant viruses deletedfor… More

There is a need for uniformly spaced duplex dna size standards (ladders) with different spacings in the range of 40 kilobase-pairs (kbp) to 10 megabase-pairs (mbp) and above for use in pulsed field gel electrophoresis. commercially available size marker (e.g. lambda-dna ladders, yeast chromosomal… More

There is a significant demand for inexpensive activated or activable gels for the coupling of antibodies and other ligands to use in affinity chromatography, industrially as well as on a laboratory scale. We will develop and optimize novel gels based on natural polymers that can be employed for this… More

Mapping of genomic dna will require the separation of very large dna molecules (ca. 0.1 to 3000 megabase-pairs (mbp)). while smaller dna molecules (0.1-20 kilobase-pairs (kbp)) are efficiently and rapidly resolved by the conventional constant field gel electrophoresis (cfge), the resolution of dna… More

Crystallization of membrane-proteins has been a difficult problem because of their peculiar physiconature. We will develop detergents that closely mimic the biological milieu inhabited by membrane prdetergents are expected to form large micelles; thereby, allowing the formation of larger, and… More