A new cell line, designated "hds", was established in a suspension culture derived from the peripheral blood of a patient with hairy cell leukemia (hcl), and the person has developed clinical resistance to alpha-interferon therapy. the patient exhibited a clinical picture characteristic of hcl,… More

Sickle cell anemia is an inherited disorder of erythrocytes characterized by chronic anemia, recurrent pain, susceptibility to infections, chronic and recurrent organ dysfunction, and diminished life-expectancy. Even among patients having documented homozygous sickle cell anemia (Hb SS), there… More

We will develop a simple chromosome band-specific painting technique, with the direct use of pcr products from microdissected defined chromosomal region as a pool of probes. the tecnique will be standardized and will be further tested during the phase ii period. after the technique/kit is fully… More

Beta-thalassemia is the most common inherited blood disorder. Advances in recombinant DNA and genetic technologies have made Beta-thalassemia the primary genetic disease of multiple mutations. Molecular basis is being characterized for more than 90% of all the defective genes and is leading to new… More

Our company will develop an in vitro tissue culture system which will permit studies of drugs'capability to enhance production of fetal hemoglobin. The Investigator stated that increased fetalhemoglobin in cells of patients with beta-hemoglobinopathy ameliorates the clinical symptoms of… More

We will develop a simple PCR method to diagnosis alpha-thalassemias. Phase I of the project willconduct feasibility study for the development of a non-isotopic sensitive assay to detect the deletionsof five frequently occurring genotypes of alpha-thalassemia using differential PCR. The Investigator… More

We will utilize conditioned media (CM) from cultures of the nasopharyngeal carcinoma (NPC) cell line CNEI. This cell line was derived from a patient in China and is the only NPC cell line that has been adapted to grow in the absence of serum. Preliminary evidence has established that the CNEI cell… More

During pregnancy small numbers of fetal erythrocytes enter the maternal circulation. Among these cells are nucleated erythrocytes called normoblasts. Genomic analysis of these normoblasts using specific probes by in situ hybridization or the polymerase chain reaction (PCR) potentially provides a… More