Combinatorial Molecular Array Analysis via MEF/MALDI/MS
DESCRIPTION (provided by applicant):
Despite numerous advances in high throughput screening (HTS) and system automation, the identification of specific ligands remains time consuming and resource intensive. A major obstacle to more efficient screening of potential ligands is the current requirement for each target protein to be screened individually. A highly effective method for identifying proteins bound to solid supports is matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). But variability in protein-matrix interactions can make accurate assessments of protein binding difficult, since the absence of mass signal could signify either the absence of binding or the poor desorption/ionization behavior of a particular protein/matrix pair. This ambiguity can be resolved, however, by employing a second, independent measure of binding that is not subject to the same protein-to-protein variability. To this end, we propose the development of a novel discovery platform for the combinatorial analysis of molecular arrays, incorporating metal enhanced fluorescence (MEF) to detect protein binding, and MALDI mass spectrometry to identify the proteins involved.
Small Business Information at Submission:
AGAVE BIOSYSTEMS BOX 80010 AUSTIN, TX 78708
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