Phage Array Biosensor for Detection of Biowarfare Agents
Agency / Branch:
DOD / ARMY
Agave BioSystems, in collaboration with Professor George Malliaras of Cornell University, proposes to develop a unique and innovative biosensor based on induced luminescence of captured BW bacterial agents and organic light emitting diode (OLED)technology. The system would use an array of bacteriophage engineered to express fluorescent protein in infected BW agents. The specificity of the phage provides capture of only targets of interest, while the infection of the bacteria and naturalreplication of the expressed protein will provide the detection signal. Using novel OLED arrays, a phage array chip can be constructed similar to DNA chips for multianalyte detection. The combination of the phage array approach with OLED detectionallows development of a powerful biosensing system that does not require additional labeling, sample manipulation, or sophisticated microfluidic and pumping mechanisms.Potential markets include the food processing, environmental, medical and agriculturalsectors. Relevant examples include the detection of Listeria monocytogenes in dairy foods and detection of multi-drug resistant bacteria in hospitals and clinics. All bacteria responsible for these outbreaks are susceptible to phage infection, and thus arelikely candidates for detection by the phage array biosensor.
Small Business Information at Submission:
Research Institution Information:
AGAVE BIOSYSTEMS, INC.
P.O. Box 80010 Austin, TX 78708
Number of Employees:
120 Day Hall
Ithaca, NY 14853
Nonprofit college or university