Novel one-step purification with a sortase fusion

Award Information

Agency: Department of Health and Human Services	Branch: N/A	Award ID: 76273	Program Year/Program: 2005 / SBIR
Agency Tracking Number: GM074422	Solicitation Year: N/A	Solicitation Topic Code: N/A	Solicitation Number: N/A

Small Business Information: Advanced Protein Labs
Advanced Protein Labs 505 Coast Blvd S, Ste 412 La Jolla, CA 92037 0461
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Woman-Owned: Yes

Minority-Owned: Yes

HUBZone-Owned: No

Phase 1: Fiscal Year: 2005

Title: Novel one-step purification with a sortase fusion

Agency: HHS

Contract: 1R43GM074422-01

Award Amount: $100,000.00

Abstract:
DESCRIPTION (provided by applicant): A new protein fusion expression system was conceptualized and is being developed to provide tag-free recombinant protein purification via one-step chromatography. Phase I of this project includes optimization of the activity of the key component in the fusion construct, streamlining the target cloning method, and applications in bacteria expression system. Because this purification method is simple, low cost, time saving, Phase II of this project will advance the one-step method for eukaryotic expression and apply the method to producing proteins for proteomic studies and biotransformation applications.

Principal Investigator:
Hongyuan Mao
8587543014
HMAO@SAN.RR.COM

Business Contact:
Hongyuan Mao
8587543014
HMAO@SAN.RR.COM

Small Business Information at Submission:

Advanced Protein Labs
Advanced Protein Labs 505 Coast Blvd S, Ste 412 La Jolla, CA 92037

EIN/Tax ID: 651245982

DUNS: N/A

Number of Employees: N/A

Woman-Owned: No

Minority-Owned: No

HUBZone-Owned: No