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NEW FLOW CYTOMETRY TECHNOLOGIES FOR CLINICAL HEMATOLOGY

Award Information

Agency:
Department of Health and Human Services 		Branch:
N/A 		Award ID:
13916 		Program Year/Program:
1993 / SBIR
Agency Tracking Number:
13916 		Solicitation Year:
N/A 		Solicitation Topic Code:
N/A 		Solicitation Number:
N/A
Small Business Information
ABIOMED, INC.
22 CHERRY HILL DRIVE DANVERS, MA 01923-
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1993
Title: NEW FLOW CYTOMETRY TECHNOLOGIES FOR CLINICAL HEMATOLOGY
Agency: HHS
Contract: N/A
Award Amount: $500,000.00
 

Abstract:

AUTOMATED BLOOD CELL ANALYSIS IS A ROUTINE FUNCTION IN THE CLINICAL HEMATOLOGY LABORATORY. HOWEVER, THE FLOW CYTOMETRIC INSTRUMENTATION PERFORMING THESE TESTS MAY HAVE FUNDAMENTAL LIMITATIONS REGARDING MEASUREMENT OF RED BLOOD CELL VOLUME, RED BLOOD CELL HEMOGLOBIN CONCENTRATION, AND ACCURATE CLASSIFICATION OF WHITE BLOOD CELL SUBPOPULATIONS. THE "HIGH-END" INSTRUMENTS HAVE BECOME VERY COMPLEX AND EXPENSIVE IN ORDER TO ENSURE THAT AN ACCURATE AND RELIABLE RESULT CAN BE REPORTED. YET, THERE IS NO TOOL AVAILABLE TO DO THESE KINDS OF MEASUREMENTS ANY DIFFERENTLY OR BETTER. THIS PROJECT IS CONCERNED WITH TWO METHODS FOR THE CLINICAL FLOW CYTOMETRY HEMATOLOGY LABORATORY THAT CAN OVERCOME THE PROBLEM ASSOCIATED WITH HEMOGLOBIN CONCENTRATION AND VOLUME INTERFERENCES WHEN MAKING RED BLOOD CELL MEASUREMENTS AND OVERCOME THE PROBLEM OF REFRACTIVE INDEX AND VOLUME INTERFERENCE IN THE WHITE BLOOD CELL DIFFERENTIAL MEASUREMENT. THIS IS DONE BY SIMULTANEOUSLY DETERMINING CELL DRY MASS BY MEASUREMENTS OF PHASE USING A TWO-FREQUENCYHENE LASER AND CELL VOLUME BY THE PRINCIPLE OF DYE EXCLUSION. THIS PHASE I RESEARCH WILL CHARACTERIZE BOTH TECHNIQUES USING PARTICLE AND BLOOD SAMPLES ON AN EXPERIMENTAL FLOW CYTOMETER. THE ULTIMATE INSTRUMENT WILL BE A LOW-COST DEVICE CAPABLE OF MAKING ACCURATE RED BLOOD CELL AND WHITE BLOOD CELL DRY MASS AND VOLUME MEASUREMENTS.

Principal Investigator:

Douglas E Burger
5087775410

Business Contact:

Small Business Information at Submission:

Abiomed Inc.
33 Cherry Hill Dr Danvers, MA 01923

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No