You are here

Establishing a neuroprotection model in the nonhuman primate to support translati

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 1R43HG007626-01
Agency Tracking Number: R43HG007626
Amount: $299,795.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: NHGRI
Solicitation Number: PA12-088
Timeline
Solicitation Year: 2013
Award Year: 2013
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
365 San Aleso Ave
Sunnyvale, CA 94085-1442
United States
DUNS: 78649664
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 ARJANG HASSIBI
 (512) 232-7916
 arjang.hassibi@insilixa.com
Business Contact
 MARK MILLER
Phone: (925) 519-5051
Email: mark.miller@insilixa.com
Research Institution
 Stub
Abstract

CMOS-Integrated Fluorescence Biochip Arrays Abstract Array technologies have been responsible for a multitude of discoveries in genomics and proteomics. However, they require sophisticated and highly accurate instrumentation. Today, the bulkiness, cost, and complexity of array readers have become barriers to their adoption in point-of-care (PoC) applications. There is thus a huge demand for rugged, portable, low cost, and ease-of-use systems that can be used outside of core facilities. In this proposal, we will develop a semiconductor-integrated solution for this problem: a CMOS-integrated fluorescence biochip. This system offers the best of both worlds by integrating the gold standard detection modality of biotechnology (fluorescence detection) with commercially available, conventional semiconductor manufacturing processes (complementary metal-oxide-semiconductor, CMOS). The active array substrates include not only the individual transducers required for sensing, but also the low-noise and high dynamic range sensor circuitry and electronic signal analysis blocks. Our preliminary results have demonstrated that by using CMOS, we will not only offer unprecedented detection dynamic range, but also make the cost of the integrated biochip arrays negligible. Thelatter is a truly unique criterion, as it justifies integration efforts by allowing the biochip array (effectively the reader) to be disposable. In this Phase I project, we plan to design and optimize a biochip system for the widely used DNA microarraysin molecular diagnostics applications with lt1000 DNA capturing spots. The integrated biochip will be capable of high performance and multicolor fluorescence detection within the visible-range ( =400nm to 800nm) with an array size of 1000 and a pixel pitchof 100 m. The specific tasks in this project will be to (1) design and fabricate the electronics circuits that are required for such CMOS biochips, (2) integrate the emission filter, (3) optimize the surface functionalization protocols, and (4) create amicroarray-compatible fluidics module for seamless experimentation. Our ultimate quantitative goal of this Phase I SBIR is to experimentally validate the whole system for DNA microarray applications and further develop and commercialize this technology inPhase II. PUBLIC HEALTH RELEVANCE PUBLIC HEALTH RELEVANCE: Point-of-care (PoC) molecular diagnostic requires highly-integrated, robust, easy-to-use, low cost, and accurate detection platforms. Semiconductor integration can offer such characteristics forgenomics and proteomics array-based screening technologies. In this project, we develop disposable CMOS-integrated fluorescence biochip arrays that can simultaneously screen 1000's of different DNA strands in real-time without requiring any bulky instrumentation or array reader whatsoever while offering unprecedented detection dynamic range.

* Information listed above is at the time of submission. *

US Flag An Official Website of the United States Government