You are here

Improving Pediatric Developmental Screening and Communication: A CME

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 1R43DA036242-01
Agency Tracking Number: R43DA036242
Amount: $199,939.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: NIDA
Solicitation Number: PA12-088
Timeline
Solicitation Year: 2013
Award Year: 2013
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
9924 Mesa Rim Road
SAN DIEGO, CA 92121-2910
United States
DUNS: 21345447
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: Yes
Principal Investigator
 JIWU WANG
 (858) 587-6645
 jiwuwang@allelebiotech.com
Business Contact
 JIWU WANG
Phone: (858) 587-6645
Email: jiwuwang@allelebiotech.com
Research Institution
 Stub
Abstract

DESCRIPTION (provided by applicant): The goal of the proposed project is to develop a commercial kit that will allow drug addiction researchers with ongoing projects using cell-based models to be able to successfully and conveniently produce human inducedpluripotent stem cells (iPSCs). Addiction to drugs destroys lives of substance abusers and their families, creating enormous social problems and exerting cost on the nation's health care system. Molecular mechanisms that contribute to the initiation and establishment of addiction are largely unknown, partially due to the lack of neural cell types from the patients. The technologies that made possible of human iPSCs opened the doors to new areas of direct interrogation of the pathophysiology of addiction byusing patient-specific cell sources. Currently, the NIDA is funding projects that rely on iPS technologies to: 1) characterize dopaminergic neurotransmission function of neurons derived from iPSCs with genomic aberrations, such as chromosomal polymorphismregions containing dopamine transporter (DAT) and vesicular monoamine transporter (VMAT2) genes; 2) identify genetic variations that can be functionally correlated to addiction in patient cell-derived neurons via iPSCs; 3) make dopamine neurons from patient iPSCs and expose them to methamphetamine and withdrawal in order to analyze the electrophysiological properties of patient-specific neurons versus controls, etc. Even though it is considered a technology with great potential in studies of addiction or other diseases, reprogramming of adult human cells to iPSCs is still an inefficient, tedious and costly process to most researchers. The currently prevailing methods of reprogramming have additional problems such as alteration of genomes or introduction of viruses that can complicate the interpretation of results from the cell-based assays. This project aims to develop a turn-key tool kit that will enable many biomedical researchers to generate iPSCs not only with 10 to 100 folds higher efficiency than othermethods, in less than 2 weeks, but also through a feeder-free, xeno-free and footprint-free procedure. PUBLIC HEALTH RELEVANCE PUBLIC HEALTH RELEVANCE: The goal of the proposed research is to develop a research tool kit for highly efficient generation of human induced pluripotent stem cells from adult cells. The technology will allow discoveries of novel therapies using stem cells or patient-specific disease models.

* Information listed above is at the time of submission. *

US Flag An Official Website of the United States Government