High-capacity and Cost-effective Manufacture of Chloroperoxidase
Agency / Branch:
DOD / ARMY
The chloroperoxidase enzyme from the filamentous fungus Caldariomyces fumago has applications in industrial chemical synthesis and the detection and inactivation of chemical warfare agents. Chloroperoxidase is capable of regio- and enantioselective oxygenations and halogenations of organic substrates. When performed chemically, these reactions typically require aggressive reagents and reaction conditions, and lead to the formation of undesired by-products. Widespread adoption of enzyme-catalyzed synthetic strategies is hindered by the high cost of purified proteins, and by the challenges of retaining the native activity of proteins expressed using heterologous host systems. Chloroperoxidase is a heavily glycosylated protein, and only when it is expressed in filamentous fungal hosts such as Aspergillus niger are the post-translational modifications necessary for its activity performed with fidelity. Development of optimized Aspergillus strains and constructs has facilitated heterologous expression of a range of secreted proteins. In this Phase I, Agave BioSystems proposes to develop a system for expression and purification of chloroperoxidase (CPO) from C. fumago using the filamentous fungus Aspergillus niger as host. Fermentation conditions will be optimized for high volume and cost-effective production, and the biochemical properties of the recombinant enzyme will be characterized.
Small Business Information at Submission:
Research Institution Information:
Agave BioSystems, Inc.
P.O. Box 100 Ithaca, NY -
Number of Employees:
12 Day Hall
Ithaca, NY 14853-