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High-capacity and Cost-effective Manufacture of Chloroperoxidase

Award Information

Agency:
Department of Defense
Branch:
Army
Award ID:
Program Year/Program:
2011 / STTR
Agency Tracking Number:
A11A-014-0164
Solicitation Year:
2011
Solicitation Topic Code:
A11a-T014
Solicitation Number:
2011.A
Small Business Information
Agave BioSystems, Inc.
P.O. Box 100 Ithaca, NY 14850-
View profile »
Woman-Owned: No
Minority-Owned: Yes
HUBZone-Owned: Yes
 
Phase 1
Fiscal Year: 2011
Title: High-capacity and Cost-effective Manufacture of Chloroperoxidase
Agency / Branch: DOD / ARMY
Contract: W911NF-11-C-0231
Award Amount: $100,000.00
 

Abstract:

The chloroperoxidase enzyme from the filamentous fungus Caldariomyces fumago has applications in industrial chemical synthesis and the detection and inactivation of chemical warfare agents. Chloroperoxidase is capable of regio- and enantioselective oxygenations and halogenations of organic substrates. When performed chemically, these reactions typically require aggressive reagents and reaction conditions, and lead to the formation of undesired by-products. Widespread adoption of enzyme-catalyzed synthetic strategies is hindered by the high cost of purified proteins, and by the challenges of retaining the native activity of proteins expressed using heterologous host systems. Chloroperoxidase is a heavily glycosylated protein, and only when it is expressed in filamentous fungal hosts such as Aspergillus niger are the post-translational modifications necessary for its activity performed with fidelity. Development of optimized Aspergillus strains and constructs has facilitated heterologous expression of a range of secreted proteins. In this Phase I, Agave BioSystems proposes to develop a system for expression and purification of chloroperoxidase (CPO) from C. fumago using the filamentous fungus Aspergillus niger as host. Fermentation conditions will be optimized for high volume and cost-effective production, and the biochemical properties of the recombinant enzyme will be characterized.

Principal Investigator:

John Ramsey
Staff Scientist
(607) 272-0002
jramsey@agavebio.com

Business Contact:

Noe Salazar
President
(512) 373-8601
nsalazar@agavebio.com
Small Business Information at Submission:

Agave BioSystems, Inc.
P.O. Box 100 Ithaca, NY -

EIN/Tax ID: 161551819
DUNS: N/A
Number of Employees:
Woman-Owned: No
Minority-Owned: Yes
HUBZone-Owned: Yes
Research Institution Information:
Cornell University
12 Day Hall
Ithaca, NY 14853-
Contact: Linda Brainard
Contact Phone: (607) 255-7123