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FP-Aptamer Reader for Algal Toxins

Award Information
Agency: Department of Commerce
Branch: N/A
Contract: DG1330-02-CN-0039
Agency Tracking Number: 2002-23
Amount: $49,989.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Timeline
Solicitation Year: N/A
Award Year: 2002
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
101 West Sixth Street Suite 200
Austin, TX 78701
United States
DUNS: N/A
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 John Bruno
 Chief Technical Officer
 (512) 479-7732
 bruno@spec.com
Business Contact
 James McNeal
Title: Contracts Director
Phone: (512) 479-7732
Email: mcneal@spec.com
Research Institution
N/A
Abstract

OmniSite BioDiagnostics (OmniSite) proposes to develop artificial receptors composed of DNA oligomers (called ¿Aptamers") for binding and detecting algal toxins. DNA aptamers can be mass produced with relative ease compared to antibody production and purification. The aptamers will be 5'-Cy 5 fluorochrome labeled and used in simple one-step binding fluorescence polarization (FP) assays that do not require wash steps. In Phase 1, OmniSite proposes to generate a set of specific fluoresceinated DNA aptamers to bind and detect Brevetoxin and Saxitoxin. Detection of the aptamer bound toxins will be accomplished via an FP breadboard system to be designed and constructed by OmniSite. In Phase 2, OmniSite will produce a hand-held FP prototype detector with wireless Internet connectivity so that test results can be accessed remotely. In addition, OmniSite will clone and sequence the anti-toxin aptamers and optimize FP aptamer assays for field use by water and seafood inspectors

* Information listed above is at the time of submission. *

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