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Parallel Microfluidic System for High Throughput Label Free Cytokine Analysis

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 1R43AI081533-01
Agency Tracking Number: AI081533
Amount: $199,776.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: PHS2007-2
Timeline
Solicitation Year: 2008
Award Year: 2008
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
4909 BROWN VILLA COVE
SALT LAKE CITY, UT 84123
United States
DUNS: 171205177
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 () -
Business Contact
Phone: (801) 792-7074
Email: bruce@microfl.com
Research Institution
N/A
Abstract

DESCRIPTION (provided by applicant): The ultimate goal of this project is to develop a multiplex label free platform for high- throughput cytokine screening of patient samples. Though there are benefits to the current suspension phase multiplex cytokine a
nalysis tools (Luminex), the technology suffers from a number of drawbacks including reagent variability, high reagent costs, and long run times. The development of a high throughput label free system will improve the throughput and reproducibility of cyto
kine analyses. In addition, cost savings will be realized through reductions in development costs and the removal of labeling reagents. Accordingly, Wasatch Microfluidics will team with the Baylor Institute of Immunological Research (BIIR) to adapt Wasatch
's continuous flow spotting technologies to create a flow cell array directly integrated with a commercial biosensor array platform. Currently, flow cell technology is the limiting factor in the development of high throughput label free sensing technologie
s. Modification of Wasatch Microfluidics Continuous Flow MicrospotterTM into a highly parallel flow cell should begin to eliminate this bottleneck and provide a template for even more highly parallel systems. Preliminary work suggests that a flow cell arra
y can convert mediocre SPR imaging instruments into highly competitive protein analysis instruments comparable to state-of-the-art SPR instruments with meager throughput. Once the flow cell is completed and integrated with the commercial biosensor platform
at the BIIR, validation testing will be conducted to demonstrate cytokine discrimination and quantitative measurement of cytokine levels in a high throughput multiplex label free format.

* Information listed above is at the time of submission. *

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