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DETECTING DNA DAMAGE IN INDIVIDUAL GERM CELLS

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 13766
Amount: $49,000.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Timeline
Solicitation Year: N/A
Award Year: 1990
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
Po Box 13501
Res Triangle Park, NC 27709
United States
DUNS: N/A
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 Raymond Tice
 (919) 544-5857
Business Contact
Phone: () -
Research Institution
N/A
Abstract

A RECENTLY DEVELOPED MICROGEL ALKALINE ELECTROPHORESIS TECHNIQUE PROVIDES AN EXTREMELY SENSITIVE METHOD FOR EVALUATING THE PRESENCE OF DNA DAMAGE (SINGLE-STRAND BREAKS AND/OR ALKALI-LABILE SITES) IN INDIVIDUAL CELLS. BECAUSE EXCISION REPAIR PROCESSES ARE NOT REQUIRED TO DEMONSTRATE THE PRESENCE OF DNA LESIONS AND BECAUSE SAMPLE SIZES CAN BE EXTREMELY SMALL (1 TO <10(5)), THE SINGLE-CELL GEL TECHNIQUESHOULD BE SUPERIOR TO OTHER METHODS CURRENTLY USED TO EVALUATE DNA DAMAGE IN GERM CELLS. FURTHERMORE, BECAUSE DATA ARE COLLECTED AT THE LEVEL OF THE SINGLE CELL, AN ASSESSMENT OF INTERCELLULAR DIFFERENCES IN DAMAGE CAN BE CONDUCTED, MAKING IT MORE INFORMATIVE THAN BIOCHEMICAL TECHNIQUES BASED ON POOLED CELL SAMPLES. THE PURPOSE OF THIS PROJECT IS TO EVALUATE THE SUITABILITY OF THIS TECHNIQUE FOR DETECTING DNA DAMAGE IN THE CELLS OF MALE MICE EXPOSED TO POSSIBLE MUTAGENS. THE FOCUS OF THE PROJECT WILL BE ON THE VARIOUS METHODS USED TO ISOLATE GERM CELLS FROM THE TESTIS AND THE KINETICS OF THE INDUCTION OF DNA DAMAGE INDUCED BY SELECTED MUTAGENIC AGENTS (ACRYLAMIDE,CIS-PLATINUM, CYCLOPHOSPHAMIDE, AND METHYLMETHANESULFONATE).

* Information listed above is at the time of submission. *

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