An Official Website of the United States Government
Generating Cloned Attenuated Dengue 1 Viral cDNA Sequences
- Small Business Information
-
QUALITY BIOLOGICAL, INC.QUALITY BIOLOGICAL, INC. 7581 LINDBERGH DRIVE GAITHERSBURG, MD 20879View profile »Woman-Owned: NoMinority-Owned: YesHUBZone-Owned: Yes
- Phase 2
-
Fiscal Year: 1995Title: Generating Cloned Attenuated Dengue 1 Viral cDNA SequencesAgency / Branch: DOD / NAVYContract: N/AAward Amount: $750,000.00
Abstract:
Wild type (wt) dengue 1 viral RNA will be used as substrate to generate a complementary DNA (cDNA) library of clones that contain identified and sequenced DNA segments spanning the entire dengue 1 genome. Point mutations designed to generate amino acid alterations will be introduced at the nucleotide level in the cloned dengue 1 DNA sequences by polymerase chain reaction amplification. These mutations will be based upon sequence alterations that have previously been identified by Naval medical researachers in non-virulent or attenuated dengue 1 strains as potentially useful for development of an attenuated dengue 1 vaccine. In Phase II, mutagenized cDNA clones will be combined with appropriate cloned parental wt dengue 1 sequences to produce full-length chimeric dengue 1 cDNAs. These full-length viral cDNAs will subsequently be transcribed into infectious dengue 1 viral RNAs containing mutations of interest for development of a live attenuated dengue 1 viral vaccine and for analysis of dengue 1 pathogenicity and immunogenicity.Small Business Information at Submission:Principal Investigator:
Sandra K. Dusing
3018409331Business Contact:
Quality Biological, Inc.
7581 Lindbergh Drive Gaithersburg, MD 20879EIN/Tax ID:DUNS: N/ANumber of Employees:Woman-Owned: NoMinority-Owned: NoHUBZone-Owned: No
Award Information
|
Agency: Department of Defense |
Branch: Navy |
Award ID: 25684 |
Program Year/Program: 1995 / SBIR |
|
Agency Tracking Number: 25684 |
Solicitation Year: N/A |
Solicitation Topic Code: N/A |
Solicitation Number: N/A |