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Generating Cloned Attenuated Dengue 1 Viral cDNA Sequences

Award Information

Agency:
Department of Defense
Branch:
Navy
Award ID:
25684
Program Year/Program:
1995 / SBIR
Agency Tracking Number:
25684
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
QUALITY BIOLOGICAL, INC.
QUALITY BIOLOGICAL, INC. 7581 LINDBERGH DRIVE GAITHERSBURG, MD 20879
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Woman-Owned: Yes
Minority-Owned: Yes
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1995
Title: Generating Cloned Attenuated Dengue 1 Viral cDNA Sequences
Agency / Branch: DOD / NAVY
Contract: N/A
Award Amount: $750,000.00
 

Abstract:

Wild type (wt) dengue 1 viral RNA will be used as substrate to generate a complementary DNA (cDNA) library of clones that contain identified and sequenced DNA segments spanning the entire dengue 1 genome. Point mutations designed to generate amino acid alterations will be introduced at the nucleotide level in the cloned dengue 1 DNA sequences by polymerase chain reaction amplification. These mutations will be based upon sequence alterations that have previously been identified by Naval medical researachers in non-virulent or attenuated dengue 1 strains as potentially useful for development of an attenuated dengue 1 vaccine. In Phase II, mutagenized cDNA clones will be combined with appropriate cloned parental wt dengue 1 sequences to produce full-length chimeric dengue 1 cDNAs. These full-length viral cDNAs will subsequently be transcribed into infectious dengue 1 viral RNAs containing mutations of interest for development of a live attenuated dengue 1 viral vaccine and for analysis of dengue 1 pathogenicity and immunogenicity.

Principal Investigator:

Sandra K. Dusing
3018409331

Business Contact:

Small Business Information at Submission:

Quality Biological, Inc.
7581 Lindbergh Drive Gaithersburg, MD 20879

EIN/Tax ID:
DUNS: N/A
Number of Employees:
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No