You are here

OLIGONUCLEOTIDE MICROARRAYS FOR GENE EXPRESSION

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: N/A
Agency Tracking Number: 1R43CA089984-01
Amount: $99,800.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Timeline
Solicitation Year: N/A
Award Year: 2001
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
1104 SUMMIT AVE, STE 110
PLANO, TX 75074
United States
DUNS: N/A
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 BOGDAN ANTOHE
 () -
Business Contact
Phone: (972) 578-8076
Email: DWALLACE@MICROFAB.COM
Research Institution
N/A
Abstract

APPLICANT?S DESCRIPTION: We will develop an ink-jet based microdispensing
system for in situ synthesis of oligonucleotides in a microarray format for
gene expression studies (expression chip). Multi-channel ink-jet devices will
be utilized to increase throughput by an order of magnitude over single
dispenser per base methods. The ink-jet based approach will: 1) allow
flexibility in oligonucleotide design, 2) ensure high-throughput manufacture
capabilities 3) provide a low cost alternative to current commercially
available oligonucleotide-based microarrays, and 4) generate microarrays in a
format that can be used with commercially available fluorescence detection
scanners/instruments.
The microarrays will facilitate the discovery of drug targets, which may
interact with the genes and/or gene products expressed during the disease
state. The toxicological and pharmacological responses to these drug leads can
also be monitored. The use of such microchips will allow medical professionals
to prescribe the most effective drugs, having the least side effects, based on
a gene expression profile of an individual. Low cost microarrays will help to
place this enabling technology into the hands of all researchers, thereby
promoting our understanding of the changes in gene expression during cellular
responses to developmental cues, physiological or pathological conditions, or
external stimuli.
PROPOSED COMMERCIAL APPLICATION:
The in situ oligonucleotide synthesizer will provide low cost custom and "off-the-shelf"
microarrays for various research applications. Data from such arrays can be used to build
a bioinformatics database of gene expression profiles of cells and tissues under various
conditions to provide for objective measurements of normal and disease states and disease
associated pathways. Gene expression microarrays will also provide essential information
for the characterization of normal versus diseased states, leading to the development of
new treatment.

* Information listed above is at the time of submission. *

US Flag An Official Website of the United States Government