This Small Business Innovation Research (SBIR) Phase I project will develop new thermostable bacteriophage and archaeaviral DNA polymerases for use as improved reagents for DNA amplification, sequence analysis and single nucleotide polymorphism (SNP) detection. This will be accomplished by direct… More

70588 Modern methods of molecular analysis reveal an astonishing discrepancy between the diversity and numbers of organisms present in natural habitats and the relatively few capable of being cultured. Single Cell Genomics is a new initiative to develop the tools and methods for extracting… More

This Small Business Innovation Research Phase II project will develop novel DNA polymerase reagents for use in current and developing DNA diagnostic procedures. The approach is to develop thermophilic phage DNA replicases in place of the currently used DNA repair enzymes. The feasibility of this… More

This Small Business Innovation Research Phase II project will develop novel DNA polymerase reagents for use in current and developing DNA diagnostic procedures. The approach is to develop thermophilic phage DNA replicases in place of the currently used DNA repair enzymes. The feasibility of this… More

DESCRIPTION (provided by applicant): This technology is intended to reduce the costs of sequencing by improving the ability to sequence through difficult templates such as G/C or A/T rich regions, direct and inverted repeats, homopolymer tracts, and di, and tri-nucleotide repeats. This will decrease… More

DESCRIPTION (provided by applicant): Large insert DNA libraries are essential for the molecular analysis of the human genome and other genomes important to our well-being. Comparative genomics reveals new information about metabolic pathways, cancer, horizontal gene transfer, evolution, protein… More

70588S02-II Less than 1% of the microbes in the environment can be cultured in the lab. Therefore, to reveal the full genetic diversity of microbial communities, this project will develop technology to clone the entire genome of a single microbial cell. The approach will allow whole genome… More

70588S02-II Less than 1% of the microbes in the environment can be cultured in the lab. Therefore, to reveal the full genetic diversity of microbial communities, this project will develop technology to clone the entire genome of a single microbial cell. The approach will allow whole genome… More

DESCRIPTION (provided by applicant): Shotgun clone libraries are essential resources for molecular analysis of the human genome and of other genomes important to our well-being. Complete genomic sequencing and comparative genomics reveal new information about metabolic pathways in normal and… More

DESCRIPTION (provided by applicant): Large insert DMA libraries are essential resources for the molecular analysis of the human genome and other genomes important to our well-being. The identification of susceptibility genes for complex disorders is critically dependent on a complete and accurate… More

DESCRIPTION (provided by applicant): Thermos table DNA polymerases are indispensable reagents in two of the most commonly used methods in health research today, DNA amplification and sequencing, as well as in SNP detection. While Taq DNA polymerase and other available enzymes are certainly… More

DESCRIPTION (provided by applicant): Unbiased and accurate cloning is essential for analysis of the human genome and other genomes important to our well-being. Complete genomic sequencing and comparative genomics reveal new information about metabolic pathways in normal tissue as well as diseased… More

DESCRIPTION (provided by applicant): DNA polymerases are the engines that drive conventional methods of DNA sequence analysis, as well as many of the newer sequencing methods being developed. Variants of Taq DNA polymerase have been used for nearly all genomic sequencing to date. However, Taq DNA… More

DESCRIPTION (provided by applicant): Functional analysis of proteins is essential to thoroughly understand the basic interactions essential for life. However, obtaining pure proteins for basic research and drug screening is limited by technical bottlenecks . For example, high throughput studies are… More

DESCRIPTION (provided by applicant): This project proposes is that deficiencies in available DNA polymerases increase costs and limit the rate of DNA sequencing and that thermophilic phage DNA polymerases developed here will substantially improve automated fluorescent sequencing, as well as newer… More

DESCRIPTION (provided by applicant): The 100 trillion microorganisms that naturally live inside and on humans are estimated to outnumber human cells by a factor of ten. The Human Microbiome Project sponsored by the NIH seeks to better understand the effect s these complex communities have on human… More

DESCRIPTION (provided by applicant): A complete and accurate collection of all expressed genes is critical for genomic and proteomic research of any genome. However, despite significant efforts to clone all the expressed human genes, approximately 20% of the predicted protein-coding genes are… More

This Small Business Innovation Research (SBIR) Phase I project proposes to improve the reliability of widely used methods for detecting and measuring trace amounts of RNA (genetic material actively made by cells and contained within certain viruses). Most current methods for detecting RNA use two… More

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5). This Small Business Innovation Research Phase I project is aimed at developing an effective, inexpensive, safe means of controlling infectious diseases among farmed catfish. An integrated system of… More

DESCRIPTION (provided by applicant): The Polymerase Chain Reaction (PCR) is one of the most widely used techniques in molecular biology. Unfortunately, thermocycling at elevated temperatures (60o to 95oC) causes extensive thermal damage to DNA; in particu lar, A+G depurination. In vivo, apurinic… More

DESCRIPTION (provided by applicant): Respiratory infections are a leading cause of morbidity and mortality and have a dramatic impact on global healthcare costs and productivity. Rapid, simple, and robust point of care (POC) detection methods is crucial to effectively combat these infections.… More

DESCRIPTION (provided by applicant): In the post-genomic era, a vast collection of protein-coding sequences is available for structural and functional analysis. Current structural genomics efforts seek to determine the complete repertoire of protein str uctures found in nature. The success of… More

DESCRIPTION (provided by applicant): The long term objective of this research is to develop improved DNA polymerases that will substantially advance Sanger and next generation sequencing technologies. The primary goal of the Phase I research was to deve lop a rapid and inexpensive method to… More

DESCRIPTION (provided by applicant): Next-generation sequencing (NGS) platforms are fundamentally altering genetic and genomic research by providing massive amounts of data in a low-cost, high-throughput format. The main drawback of existing technologies i s the short sequence read lengths they… More

DESCRIPTION (provided by applicant): There is societal need for new antibiotic compounds in our arsenal of defenses against bacterial pathogens, many of which are increasingly resistant to existing antibiotics. The best possible source for new antibiotic s tructures with potentially novel mechanisms… More

DESCRIPTION (provided by applicant): G-Protein coupled receptors (GPCRs) are a large family of proteins expressed on the surface of eukaryotic cells. They are the major pathway for transducing extracellular signals into a diversity of intracellular respons es. Because of their accessibility and… More

DESCRIPTION (provided by applicant): The goal of this project is to integrate an isothermal amplification system with lateral flow detection to produce a simple, sensitive, low cost, all-in-one detection device for flaviviruse that is easily performed and interpreted in a point of care setting and… More

DESCRIPTION (provided by applicant): The goal of this Phase II proposal is to develop systems for transient, stable, and transgenic expression of nearly any DNA sequence, particularly those that are impossible to clone into current vectors. Primary tar gets include genes containing long… More

This Small Business Innovation Research (SBIR) Phase II project is aimed at developing an effective, inexpensive, safe means of controlling diseases in farm-raised catfish. One of the biggest problems in farm-raised catfish is disease that occurs in commercial ponds. Scientists at Auburn University… More

DESCRIPTION (provided by applicant): Microfluidic systems are emerging as critically important tools for high throughput cell based analysis for applications including genotyping, sequencing, gene expression analysis, nucleic acid amplification, and diagnostics. Lab-on-a-chip devices that are… More

DESCRIPTION (provided by applicant): The regulation of gene expression drives all biological processes. A thorough understanding of the mechanisms governing the patterns of gene expression is necessary to understand the fundamentals of biology and the complexities of cancers and other diseases. The… More

DESCRIPTION (provided by applicant): There is societal need for new therapeutic agents in our arsenal of defenses against bacterial and fungal pathogens, many of which are increasingly resistant to existing antibiotics. Filamentous fungi are considered promising resources for the development of… More

DESCRIPTION (provided by applicant): Respiratory infections have a significant health and economic impact worldwide. Current test products for the diagnosis of respiratory viral infections such as influenza are inadequate for the timely diagnosis needed for successful implementation of antiviral… More

DESCRIPTION (provided by applicant): 100,000 Americans perish each year due to untreatable bacterial infections. The societal benefits of new antibiotic compounds that are effective against numerous multiple drug resistant pathogens would be significant. The best possible source for new antibiotic… More

DESCRIPTION (provided by applicant): A major goal in the post-genomic era is to express the vast collection of protein- coding sequences, eventually resulting in a better understanding of protein interactions and the development of novel therapeutics. Themost favored host for heterologous… More

DESCRIPTION (provided by applicant): Each year, 100,000 Americans perish due to untreatable bacterial infections. The societal benefits of new antibiotic compounds that are effective against numerous multiple drug resistant pathogens would be significant.The best possible source for new antibiotic… More

DESCRIPTION (provided by applicant): Robust expression of multi-subunit protein complexes is crucial for understanding the basic biology of organisms. Expression of the individual components in E. coli or yeast may be quick and cost-efficient, but many mammalian proteins show poor expression or… More