Genomics programs are discovering a large number of novel genes thus creating a need to express new proteins for therapeutics, diagnostics and to understand protein structure and function. LifeSensors proposes the creation of a new ubiquitin-fusion system to address this need. This proposal makes… More

"The goal of this proposal is to demonstrate the feasibility to express in a short period of time a large number of malarial antigens by using ubiquitin fusion technology. We will design the yeast expression vectors for rapid cloning of a vast number ofmalarial genes. N-terminal of malarial antigens… More

DESCRIPTION: (Provided by Applicant) Malaria continues to be one of the devastating diseases affecting a large population of the world and claims an estimated one to two million lives annually. Unfortunately, there is no vaccine available against malaria. Current… More

DESCRIPTION (provided by applicant): As more proteins are identified from the genome-sequencing projects, there is an increasing need for reliable and cost efficient means for expression and purification of proteins of academic and clinical importance. LifeSensors has developed a novel SUMO-fusion… More

DESCRIPTION (provided by applicant): A vast number of novel proteins discovered from genome sequencing projects are creating enormous opportunities for scientists in academia and industry. Large-scale consortia and initiatives are under way to understand the structure of newly discovered proteins.… More

DESCRIPTION (provided by applicant): Recombinant proteins are notorious for their short half-lives and reduced bioavailability. As more novel therapeutic proteins are identified from human genome-sequencing projects, there is an increasing need to formulate protein delivery strategies to prolong… More

DESCRIPTION (provided by applicant): A vast number of novel proteins discovered from genome sequencing projects are creating enormous opportunities for scientists in academia and industry. Large-scale consortia and initiatives are underway to understand the structures of newly discovered proteins. … More

DESCRIPTION (provided by applicant): Membrane proteins have crucial roles in many cellular and physiological processes. Understanding the structures and functions of these proteins is academically and commercially important. However, the lack of efficient methods for expressing membrane proteins has… More

DESCRIPTION (provided by applicant): As more proteins are identified from the genome-sequencing projects, there is an increasing need for reliable and cost efficient means for expression and purification of proteins of academic and clinical importance. LifeSensors has developed a novel SUMO-fusion… More

DESCRIPTION (provided by applicant): The "bottleneck" for structural and functional Genomics and Proteomics is efficient expression and purification of the proteins of interest. Advances in fusion systems have played a major role in enhancing expression of proteins. However, in many cases, using a… More

DESCRIPTION (provided by applicant): Bioinformatics tools will be generated for a class of ~ 100 proteins known as de- ubiquitinating enzymes (DUBs), which play an important role in cell survival, proliferation, and differentiation. LifeSensor's proprietar y SUMO expression enhancement technology… More

DESCRIPTION (provided by applicant): The first step in any protein therapeutic exercise is expression of the protein. In some cases, this step is not troublesome. For increasing numbers of designer proteins and requirements for post-translation modificatio ns, however, enhanced expression and… More

DESCRIPTION (provided by applicant): Rather modest success has yet been achieved in expressing and purifying membrane proteins as a prerequisite to obtain high resolution structures. Only 124 unique structures for membrane proteins have been resolved out o f gt 30,000 soluble proteins. This … More

DESCRIPTION (provided by applicant): It has been well established that the ubiquitin pathway plays an important role as a key regulator of neuronal biology. The ubiquitin pathway enzymes are essential and critical mediators of synaptic adaptability in matu re neurons, and dysfunctional ubiquitin… More

DESCRIPTION (provided by applicant): A major bottleneck in protein expression and purification is the costs associated with functional protein production in biomedical, biopharmaceutical and other research fields. Because proteins of interest originating f rom eukaryotic organisms often require… More

DESCRIPTION (provided by applicant): Bioinformatics and biochemical analysis suggests that the human genome encodes ~ 100 proteins known as deubiquitinating enzymes (DUBs). DUBs constitute an important class of enzymes that deconjugate ubiquitin and ubiqui tin-like proteins (UBL) from their target… More

DESCRIPTION (provided by applicant): As the prominence of the ubiquitin and ubiquitin-like pathways increases the need for assays to measure the activity of the enzymes involved in these pathways grows. Currently the only high throughput methods for measur ing ubiquitin and ubiquitin-like… More

DESCRIPTION (provided by applicant): The network of enzymes involved in the ubiquitination (ligases) and deubiquitination (deubiquitinases or DUbs) of cellular proteins is one of the largest in the human proteome and plays a major role in the regulation of cellular homeostasis. This network… More

DESCRIPTION (provided by applicant): In the last decade there has been an explosive growth in the field of ubiquitin research, with approximately 530 human genes predicted to encode enzymes involved in the conjugation and deconjugation of ubiquitin. Of these 95 encode for deubiquitylases (DUBs). In… More

DESCRIPTION (provided by applicant): Identification, quantification, and isolation of low abundance proteins from complex mixtures is, at best, a difficult task. This is especially the case for proteins carrying a post-translational modification (PTM) that affects their half-life or regulatory… More