You are here

DNA POLYMERASES FOR SINGLE MOLECULE DNA SEQUENCING

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: N/A
Agency Tracking Number: 1R43HG002292-01
Amount: $100,000.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Timeline
Solicitation Year: N/A
Award Year: 2001
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
BOX 4425, 4421 SUPERIOR ST
LINCOLN, NE 68504
United States
DUNS: N/A
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 JOHN WILLIAMS
 () -
Business Contact
Phone: (402) 467-3576
Email: LYLEM@LICOR.COM
Research Institution
N/A
Abstract

APPLICANT'S DESCRIPTION: DNA polymerases with altered substrate specificity are
needed to support a new approach to single molecule DNA sequencing. Single
molecule methods can be applied to DNA isolated directly from a subject
organism, eliminating the need to clone, map and sort DNA fragments prior to
sequencing. The need for electrophoretic separation is eliminated and reagent
consumption is minimal. Single molecule detection has been developed and
practiced by physicists and chemists for over 10 years. The crucial optical and
detection systems needed for single molecule sequencing now exist. To implement
this technology, novel nucleotides labeled at two different atomic positions
have been synthesized and tested as substrates for DNA polymerases. Whereas
some enzymes utilize nucletides labeled at the first position and other enzymes
utilize nucleotides labeled at the second position, we could not identify any
natural polymerase able to efficiently incorporate nucleotides labeled at both
positions (double-labeled nucleotides). In Phase I, we will develop methods for
the directed evolution of DNA polymerases adapted to the utilization of
double-labeled nucleotides. Directed evolution will be used in Phase II to
select for highly processive DNA polymerases having the desired substrate
specificities.
PROPOSED COMMERCIAL APPLICATION:
A genomic DNA sequencing technology that reduces cost and eliminates the need for
cloning, physical mapping and electrophoresis would have great appeal to both the
academic and industrial genomic research communities.

* Information listed above is at the time of submission. *

US Flag An Official Website of the United States Government