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DNA polymerase for single molecule DNA sequencing
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Email: LYLEM@LICOR.COM
DESCRIPTION (provided by applicant): We are developing a new technology for
single molecule DNA sequencing based on the incorporation of dNTPs labeled on
the g-phosphate with fluorescent dyes. The label is cleaved from the nucleotide
along with pyrophosphate when the nucleotide is incorporated into DNA by a
polymerase. The released label is sorted from intact g-dNTPs in a microfluidics
system and is identified in real time by single molecule fluorescence detection
optics. In this proposal, we develop a novel nucleotide chemistry to enable the
sorting scheme and we use directed evolution methods to adapt polymerases for
utilization of the g-dNTPs. Single molecule sequencing promises to reduce the
cost of DNA sequencing by several orders of magnitude compared to current
methods.
PROPOSED COMMERCIAL APPLICATIONS:
A genomic DNA sequencing technology that reduces cost and eliminates the need for cloning, physical mapping and electrophoresis would have great appeal to both the academic and industrial genomic research communities.
* Information listed above is at the time of submission. *