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DNA polymerase for single molecule DNA sequencing

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: N/A
Agency Tracking Number: 2R44HG002292-02
Amount: $0.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Timeline
Solicitation Year: N/A
Award Year: 2001
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
BOX 4425, 4421 SUPERIOR ST
LINCOLN, NE 68504
United States
DUNS: N/A
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 JOHN WILLIAMS
 () -
Business Contact
Phone: (402) 467-3576
Email: LYLEM@LICOR.COM
Research Institution
N/A
Abstract

DESCRIPTION (provided by applicant): We are developing a new technology for
single molecule DNA sequencing based on the incorporation of dNTPs labeled on
the g-phosphate with fluorescent dyes. The label is cleaved from the nucleotide
along with pyrophosphate when the nucleotide is incorporated into DNA by a
polymerase. The released label is sorted from intact g-dNTPs in a microfluidics
system and is identified in real time by single molecule fluorescence detection
optics. In this proposal, we develop a novel nucleotide chemistry to enable the
sorting scheme and we use directed evolution methods to adapt polymerases for
utilization of the g-dNTPs. Single molecule sequencing promises to reduce the
cost of DNA sequencing by several orders of magnitude compared to current
methods.
PROPOSED COMMERCIAL APPLICATIONS:
A genomic DNA sequencing technology that reduces cost and eliminates the need for cloning, physical mapping and electrophoresis would have great appeal to both the academic and industrial genomic research communities.

* Information listed above is at the time of submission. *

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