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UREASE-LINED IMMUNOASSAY REAGENT STABILITY STUDIES

Award Information
Agency: Department of Defense
Branch: Army
Contract: N/A
Agency Tracking Number: 15862
Amount: $901,147.00
Phase: Phase II
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Timeline
Solicitation Year: N/A
Award Year: 1992
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
99-193 Aiea Heights Dr
Aiea, HI 96701
United States
DUNS: N/A
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 Dr T J G Raybould
 Principal Investigator
 (808) 486-5381
Business Contact
Phone: () -
Research Institution
N/A
Abstract

UREASE OFFERS DISTINCT ADVANTAGES OVER OTHER ENZYMES FOR PREPARATION OF CONJUGATES USED IN ENZYME-LINKED IMMUNO-SORBENT ASSAY SYSTEMS. MOLECULAR DEVICES CORPORATION (MENLO PARK, CA) HAS CAPITALIZED ON THESE ADVANTAGES IN THEIR THRESHOLD SYSTEM. THIS IMPRESSIVE SYSTEM UTILIZES AVIDIN-BIOTIN TO "CAPTURE" ANALYTE-BOUND ANTIBODY-UREASE CONJUGATE COMPLEXES ONTO A MEMBRANE, WHERE THEY ARE QUANTITATED BY A SENSOR THAT MONITORS THE FORMATION OF PRODUCT FROM THE UREASE-UREA REACTION. THE THRESHOLD SYSTEM IS OF INTEREST TO THE U.S. ARMY FOR A RANGE OF APPLICATIONS, BUT TO BE OF VALUE AS A FIELD DETECTOR, THE UREASE CONJUGATES REQUIRED MUST BE STABLE TO STORAGE AT ELEVATED TEMPERATURES FOR UP TO FIVE YEARS. METHODS WILL THEREFORE BE INVESTIGATED FOR PRODUCING HIGH ACTIVITY UREASE-ANTIBODY CONJUGATES THAT ARE STABLE TO SHORT TERM STORAGE AT 37 DEG C. PARAMETERS WILL THEN BE INVESTIGATED THAT AFFECT LYOPHILIZATION OF SELECTED UREASEANTIBODY CONJUGATES, AND THEIR STABILITY TO SHORT TERM STORAGE AT 60 DEG C. IT IS PROPOSED THAT ONE MONOCLONAL AND ONE POLYCLONAL MODEL SYSTEM ANTIBODY BE STUDIED. THE DATA OBTAINED FROM THESE PHASE I STUDIES WILL PROVIDE VALUABLE INFORMATION ON PROMISING AVENUES OF APPROACH FOR PHASE II, WHEN FURTHER REFINEMENTS WILL ENABLE PRODUCTION OF HIGH ACTIVITY, STABLE CONJUGATES, FOR USE IN U.S. ARMY APPLICATIONS OF THE THRESHOLD SYSTEM.

* Information listed above is at the time of submission. *

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