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Nanocrystal FRET Probes for Multi-Wavelength Analysis of Protein Dynamics
Title: Principal Investigator
Phone: (540) 552-5128
Email: submissions301@lunainnovations.com
Title: Director of Contracts
Phone: (540) 558-1695
Email: submissions301@lunainnovations.com
Luna Innovations will demonstrate the feasibility of using a novel set of nanoparticle fluorescence resonant energy transfer (FRET) probes to monitor protein dynamics via real-time single-molecule spectroscopy. The proximity-dependent FRET-like interaction between nanoparticles would translate into time-dependent spectroscopic monitoring of changes in a protein molecule’s shape. The nanoparticle emitters will meet the following probe constraints: fluorescence FWHM less than 10 nm, probe diameter less than 3 nm, photoluminescence quantum yield (PLQY) greater than 90%, suspension in water, conjugation with antibodies. Strategies for optimizing the synthesis of these nanoparticles specifically for this application will be reviewed.
* Information listed above is at the time of submission. *