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Nanocrystal FRET Probes for Multi-Wavelength Analysis of Protein Dynamics

Award Information
Agency: Department of Defense
Branch: Defense Advanced Research Projects Agency
Contract: W31P4Q-07-C-0019
Agency Tracking Number: 06SB2-0070
Amount: $98,948.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: SB062-004
Solicitation Number: 2006.2
Timeline
Solicitation Year: 2006
Award Year: 2006
Award Start Date (Proposal Award Date): 2006-12-14
Award End Date (Contract End Date): 2007-08-14
Small Business Information
2851 Commerce Street
Blacksburg, VA 24060
United States
DUNS: 627132913
HUBZone Owned: Yes
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 Bryan Dickerson
 Principal Investigator
 (540) 552-5128
 submissions301@lunainnovations.com
Business Contact
 Michael Pruzan
Title: Director of Contracts
Phone: (540) 558-1695
Email: submissions301@lunainnovations.com
Research Institution
N/A
Abstract

Luna Innovations will demonstrate the feasibility of using a novel set of nanoparticle fluorescence resonant energy transfer (FRET) probes to monitor protein dynamics via real-time single-molecule spectroscopy. The proximity-dependent FRET-like interaction between nanoparticles would translate into time-dependent spectroscopic monitoring of changes in a protein molecule’s shape. The nanoparticle emitters will meet the following probe constraints: fluorescence FWHM less than 10 nm, probe diameter less than 3 nm, photoluminescence quantum yield (PLQY) greater than 90%, suspension in water, conjugation with antibodies. Strategies for optimizing the synthesis of these nanoparticles specifically for this application will be reviewed.

* Information listed above is at the time of submission. *

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