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Soil Inhibition Resistant Mutants of Taq DNA Polymerase
Award Information
Agency: Department of Agriculture
Branch: N/A
Contract: 2006-33610-17604
Agency Tracking Number: 2005-00194
Amount:
$290,400.00
Phase:
Phase II
Program:
SBIR
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Timeline
Solicitation Year:
N/A
Award Year:
2006
Award Start Date (Proposal Award Date):
N/A
Award End Date (Contract End Date):
N/A
Small Business Information
1508 South Grand Blvd.
St. Louis, MO
63104
United States
DUNS:
N/A
HUBZone Owned:
No
Woman Owned:
No
Socially and Economically Disadvantaged:
No
Principal Investigator
Name: Milko Kermekchiev
Phone: (314) 771-5566
Email: milko@klentaq.com
Phone: (314) 771-5566
Email: milko@klentaq.com
Business Contact
Name: Wayne Barnes
Title: President
Phone: (314) 680-0575
Email: wayne@klentaq.com
Title: President
Phone: (314) 680-0575
Email: wayne@klentaq.com
Research Institution
N/A
Abstract
Gene detection in soil using the Polymerase Chain Reaction (PCR) is nearly impossible due to the inhibitory affects of the components of soil, including humic acid. Currently, the DNA must be extracted from the soil in order to perform PCR. The DNA extraction/purification step is expensive and time consuming. We intend to develop commercially available PCR enzymes that will enable PCR to detect pathogens directly in soil, eliminating the extraction/purification step entirely.
* Information listed above is at the time of submission. *