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Company Information:

Name: DNA Polymerase Technology, Inc.
Address: 1508 S. Grand Blvd.
Saint Louis, MO 63104-1364
Located in HUBZone: Yes
Woman-Owned: No
Minority-Owned: No
URL: N/A
Phone: (314) 707-4919

Award Totals:

Program/Phase Award Amount ($) Number of Awards
SBIR Phase I $641,570.00 7
SBIR Phase II $2,157,835.00 4
STTR Phase I $154,502.00 1

Award List:

N/A

Award Year / Program / Phase: 2000 / STTR / Phase I
Agency: HHS
Research Institution: Washington University
Principal Investigator:
Award Amount: $154,502.00
RI Contact: N/A

TOWARDS A HEAT-LABILE CHELATOR FOR HOT-START PCR

Award Year / Program / Phase: 2001 / SBIR / Phase I
Agency: HHS
Principal Investigator: Shannon J. Flynn
Award Amount: $107,856.00
Abstract:
DESCRIPTION (applicant's abstract): Many PCR analyses, particularly those with rare targets or those with compromised DNA templates (such as forensic analyses), benefit from a hot start. In a normal (room temperature) start, the primers can prime at non-specific sequences,… More

COLD-SENSITIVE MUTANTS OF TAQ DNA POLYMERASE

Award Year / Program / Phase: 2002 / SBIR / Phase II
Agency: HHS
Principal Investigator: Milko B. Kermekchiev
Award Amount: $689,155.00
Abstract:
DESCRIPTION (provided by applicant): A hot start is beneficial or necessary for efficiency and reliability of a large portion of PCR reactions. The most demanding PCR reactions are especially dependent on a hot start. A heat switch can be added to PCR protocols by any of several manual or built-in… More

COLD-SENSITIVE MUTANTS OF TAQ DNA POLYMERASE

Award Year / Program / Phase: 2002 / SBIR / Phase I
Agency: HHS
Principal Investigator: Milko B. Kermekchiev
Award Amount: $0.00
Abstract:
DESCRIPTION (provided by applicant): A hot start is beneficial or necessary for efficiency and reliability of a large portion of PCR reactions. The most demanding PCR reactions are especially dependent on a hot start. A heat switch can be added to PCR protocols by any of several manual or built-in… More

REVERSIBLE MAGNESIUM PRECIPITATION FOR HOT-START PCR

Award Year / Program / Phase: 2002 / SBIR / Phase I
Agency: HHS
Principal Investigator: Katherine R. Rowlyk
Award Amount: $100,000.00
Abstract:
DESCRIPTION (provided by applicant): We have been investigating strategies for novel methods of hot start for PCR. One such strategy involves the sequestering of a critical component of the reaction until the reaction temperature is sufficient to ensure selective annealing of PCR primers. We have… More

Soil Inhibition Resistant Mutants of Taq DNA Polymerase

Award Year / Program / Phase: 2005 / SBIR / Phase I
Agency: USDA
Principal Investigator: Milko B. Kermekchiev
Award Amount: $78,160.00
Abstract:
Detection of microbes directly in soil is difficult. The purpose of this study is to identify enzymes which will enable gene detection direclty in crude soil samples.

Blood Inhibition Resistant Mutants of Taq DNA Polymerase

Award Year / Program / Phase: 2005 / SBIR / Phase I
Agency: HHS
Principal Investigator: Milko B. Kermekchiev
Award Amount: $107,144.00
Abstract:
DESCRIPTION (provided by applicant): The success and sensitivity of DNA detection is limited in important clinical, forensic, and environmental applications due to the presence of complex biological samples, such as blood, which reduce amplification efficiency. Different pre-PCR treatments that are… More

Soil Inhibition Resistant Mutants of Taq DNA Polymerase

Award Year / Program / Phase: 2006 / SBIR / Phase II
Agency: USDA
Principal Investigator: Milko B. Kermekchiev
Award Amount: $290,400.00
Abstract:
Gene detection in soil using the Polymerase Chain Reaction (PCR) is nearly impossible due to the inhibitory affects of the components of soil, including humic acid. Currently, the DNA must be extracted from the soil in order to perform PCR. The DNA extraction/purification step is expensive and time… More

Blood Inhibition Resistant Mutants of Taq DNA Polymerase

Award Year / Program / Phase: 2006 / SBIR / Phase II
Agency: HHS
Principal Investigator: Milko B. Kermekchiev
Award Amount: $678,280.00
Abstract:
DESCRIPTION (provided by applicant): The sensitivity of gene detection in blood specimens in important clinical and forensic PCR applications is limited due to inhibitory blood substances which reduce amplification efficiency and may lead to false negative results. Different pre-PCR treatments that… More

SBIR Phase I: Inhibition-Resistant DNA Polymerases and Other Improvements for Detection of Food-borne Pathogens.

Award Year / Program / Phase: 2009 / SBIR / Phase I
Agency: NSF
Principal Investigator: Milko Kermekchiev, DSc
Award Amount: $142,505.00
Abstract:
This Small Business Innovation Research (SBIR) Phase I project proposes the improvement of real-time PCR, a DNA-based rapid-detection method, for detection of food-borne pathogens such as Salmonella. Current methods are inadequate to accurately detect pathogens in foods such as chocolate,… More

Direct RT-PCR detection of RNA pathogens and mRNA expression in crude samples

Award Year / Program / Phase: 2010 / SBIR / Phase I
Agency: HHS
Principal Investigator: Zhian Zhang
Award Amount: $105,905.00
Abstract:
DESCRIPTION (provided by applicant): We propose to develop a highly simplified and improved method of detecting RNA for use in clinical tests and for scientific research by enabling the RT-PCR amplification of nucleic acids directly in whole blood, serum, plasma, and cell lysates. We propose a dual… More

SBIR Phase II: Inhibition-Resistant DNA Polymerases and Other Improvements for Detection of Food-borne Pathogens.

Award Year / Program / Phase: 2011 / SBIR / Phase II
Agency: NSF
Principal Investigator: Katherine Rowlyk
Award Amount: $500,000.00
Abstract:
This Small Business Innovation Research (SBIR) Phase II project proposes the development of novel enzymes (DNA polymerases) and other improvements for rapid detection of food-borne pathogens by DNA detection and amplification (PCR). PCR is a very fast and accurate method of pathogen detection,… More