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WE PROPOSE TO IDENTIFY THE GENES FOR AUTOIMMUNE DISEASE-SPECIFIC PROTEIN ANTIGENS BY THE USE OF RECOMBINANT DNA TECHNIQUES.

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 2947
Amount: $50,000.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Timeline
Solicitation Year: N/A
Award Year: 1985
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
128 Spring Street
Lexington, MA 02173
United States
DUNS: N/A
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 DONALD WARREN BOWDEN
 PRINCIPAL INVESTIGATOR
 (617) 861-9700
Business Contact
Phone: () -
Research Institution
N/A
Abstract

WE PROPOSE TO IDENTIFY THE GENES FOR AUTOIMMUNE DISEASE-SPECIFIC PROTEIN ANTIGENS BY THE USE OF RECOMBINANT DNA TECHNIQUES. THE SYSTEM THAT WE ARE USING LENDS ITSELF TO HIGH-LEVEL EXPRESSION OF THESE ANTIGENS AS HYBRID PROTEINS IN E. COLI. THIS APPROACH FACILITATES PRODUCTION OF THESE ANTIGENS FOR THE PURPOSE OF ANTIGEN CHARACTERIZATION, GENERATION OF ANTIBODIES AND DEVELOPMENT OF IMMUNODIAGNOSTIC TESTS. A CDNA LIBRARY WILL BE CONSTRUCTED FROM HUMAN PANCREAS TISSUE. CLONING WILL BE INTO THE LAMBDA GT11 SYSTEM. CLONES CARRYING ISLET CELL ANTIGEN SEQUENCES WILL BE IDENTIFIED BY DIFFERENTIAL IMMUNOSCREENING WITH SERA FROM TYPE I DIABETES PATIENTS AND SERA FROM NORMAL INDIVIDUALS. WITH THE LAMBDA GT11 SYSTEM, CLONES CARRYING DIABETES-ASSOCIATED SEQUENCES CAN BE INDUCED TO EXPRESS SUBSTANTIAL AMOUNTS OF THE PANCREAS ANTIGEN FUSED TO BETA-GALACTOSIDASE (HYBRID PROTEIN). BECAUSE OF THEIR LARGE SIZE, THESE PROTEINS CAN SUBSEQUENTLY BE EASILY PURIFIED. THE GOAL OF THIS WORK IS TO CLONE, EXPRESS AND CHARACTERIZE ISLET CELL ANTIGEN GENES AND TO DEVELOP A SIMPLE TEST FOR ISLET CELL ANTIBODIES USING ANTIGENS PRODUCED BY RECOMBINANT DNA METHODS. THE USE OF RECOMBINANT DNA TECHNIQUES GIVES THIS METHODOLOGY THE POWER TO IDENTIFY SPECIFIC DISEASE-ASSOCIATED EPITOPES. THIS WILL FACILITATE THE DEVELOPMENT OF A

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