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A Novel Microfluidic Device for Fully Automated Extraction of RNA from Cell Cultures

Award Information
Agency: National Aeronautics and Space Administration
Branch: N/A
Contract: NNJ05JA04C
Agency Tracking Number: 035706
Amount: $599,903.00
Phase: Phase II
Program: SBIR
Solicitation Topic Code: B2.03
Solicitation Number: N/A
Timeline
Solicitation Year: 2003
Award Year: 2005
Award Start Date (Proposal Award Date): 2004-11-22
Award End Date (Contract End Date): 2006-11-21
Small Business Information
215 Wynn Dr., 5th Floor
Huntsville, AL 35805-1944
United States
DUNS: 18516
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 Shankar Sundram
 Principal Investigator
 (256) 726-4892
 sxs@cfdrc.com
Business Contact
 Jennifer Swann
Title: Business Official
Phone: (256) 726-4858
Email: jls@cfdrc.com
Research Institution
N/A
Abstract

Obtaining high quality, intact RNA from cells is an ubiquitous need in the pursuit of space biology. Our overall objective is to develop and commercialize a microfluidics based miniaturized platform (MED-RNA) that can fully automate the complex process of RNA isolation. Starting from harvested whole mammalian cells in a culture medium, MED-RNA will lyse, capture, and isolate RNA content for later analysis, in a fully integrated fashion with minimal user intervention. In addition to higher yields and faster process times, losses and contamination will be minimized as a result of the miniaturization and automation. A novel and unique plastic card based fabrication technology from Micronics Corp. will be leveraged for low-cost microfabrication.

The Phase I study successfully demonstrated concept development and design of three critical aspects of the MED-RNA, (1) on-card reagent based cell lysis, (2) non-toxic electric field driven cell lysis, and (3) RNA capture and elution in a microfluidic extraction chamber. Physical prototypes of the components were fabricated and experimentally demonstrated. During Phase II, we will further optimize the individual component designs. Optimal components, along with necessary valving will be integrated on a plastic, microfluidic lab card. The fully integrated prototype lab card will be demonstrated on chosen cell lines. Further ensuring success, CFDRC has assembled an experienced, multidisciplinary team.

* Information listed above is at the time of submission. *

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