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QUANTITATIVE IND-1 MICROBEAD STANDARD IN FLOW CYTOMETRY

Award Information

Agency:
Department of Health and Human Services 		Branch:
N/A 		Award ID:
11249 		Program Year/Program:
1989 / SBIR
Agency Tracking Number:
11249 		Solicitation Year:
N/A 		Solicitation Topic Code:
N/A 		Solicitation Number:
N/A
Small Business Information
CARIBBEAN MICROPARTICLES CORP.
BOX 194344 SAN JUAN, PR 00919
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1989
Title: QUANTITATIVE IND-1 MICROBEAD STANDARD IN FLOW CYTOMETRY
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

INTRACELLULAR CALCIUM CONCENTRATION IS KNOWN TO PLAY A SIGNIFICANT ROLE IN BOTH PHYSIOLOGICAL AND PATHOPHYSIOLOGICAL CONDITIONS. RECENTLY, MUCH ATTENTION HASBEEN GIVEN TO THE DETERMINATION OF FREE INTRACELLULAR CALCIUM CONCENTRATION USING FLOW CYTOMETRY. NEWLY DEVELOPEDFLUORESCENT PROBES, SUCH AS INDO-1 WHICH EXHIBITS A LARGE SPECTRAL EMISSION SHIFT WHEN IT CHELATES CA(++) IONS, HAVE BEEN INSTRUMENTAL IN THE DEVELOPMENT OF THIS APPLICATION. HOWEVER, BECAUSE CALIBRATION OF THE FLOW CYTOMETER RESPONSE WITH RESPECT TO THE RATIO OF THE FL1 (405-NM PEAK) AND THE FL2 (485-NM PEAK) IS EXTREMELY DIFFICULT AND TIME CONSUMING,THE MEASUREMENT OF CYTOSOLIC CA(++) BY FLOW CYTOMETRY HAS BEEN LIMITED. THIS PROJECT FOCUSES ON THE DEVELOPMENT OF AN INDO-1 MICROBEAD STANDARDS KIT CONSISTING OF INDO-1 COATED MICROBEADS SUSPENDED IN A SERIES OF SOLUTIONS CONTAINING PREDETERMINED CONCENTRATIONS OF FREE CA(++). USING THIS KIT, THE RATIO OF THE FL1 AND FL2 EMISSION FLUORESCENCE OF INDO-1 MICROBEADS CAN BE DIRECTLY CORRELATED WITH FREE CALCIUM CONCENTRATION. SUCH A KIT WILL NOT ONLY MAKE CALIBRATION OF FLOW CYTOMETERS MORE CONVENIENT, BUT WILL ALSO HELP TO NORMALIZE DATA OVER TIME AND AMONG LABORATORIES. A MORE ACCURATE DETERMINATION OF CYTOSOLIC CALCIUM CONCENTRATION WILL UNDOUBTEDLY IMPROVE UNDERSTANDINGOF THE BIOLOGICAL RELEVANCE OF ALTERATIONS IN INTRACELLULAR CALCIUM CONCENTRATION.

Principal Investigator:


0

Business Contact:

Small Business Information at Submission:

Caribbean Microparticles Corp
304 Muno-rivera Ave #7 San Juan, PR 00901

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No