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THE OBJECTIVE OF THIS PROJECT IS TO FORMULATE DEFINED SERUM-FREE MEDIA THAT CAN…

Award Information

Agency:
Department of Health and Human Services 		Branch:
N/A 		Award ID:
3150 		Program Year/Program:
1985 / SBIR
Agency Tracking Number:
3150 		Solicitation Year:
N/A 		Solicitation Topic Code:
N/A 		Solicitation Number:
N/A
Small Business Information
Biological Research Faculty &
10075-20 Tyler Pl Ijamsville, MD 21754
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Woman-Owned: No
Minority-Owned: Yes
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1985
Title: THE OBJECTIVE OF THIS PROJECT IS TO FORMULATE DEFINED SERUM-FREE MEDIA THAT CAN SUSTAIN THE GROWTH OF PRIMARY AND/OR EARLY PASSAGE RODENT EMBRYO CELLS THAT ARE COMMONLY USED IN STUDIES ON THE MECHANISMS OF CARCINOGENESIS AS WELL AS IN THE DEVELOPMENT OF
Agency: HHS
Contract: N/A
Award Amount: $49,988.00
 

Abstract:

THE OBJECTIVE OF THIS PROJECT IS TO FORMULATE DEFINED SERUM-FREE MEDIA THAT CAN SUSTAIN THE GROWTH OF PRIMARY AND/OR EARLY PASSAGE RODENT EMBRYO CELLS THAT ARE COMMONLY USED IN STUDIES ON THE MECHANISMS OF CARCINOGENESIS AS WELL AS IN THE DEVELOPMENT OF GENETIC TOXICOLOGY ASSAYS. PHASE I STUDIES INVOLVE: A. SELECTION OF A BASE MEDIUM THAT WOULD GIVE A MAXIMUM PROLIFERATIVE POTENTIAL TO SYRIAN HAMSTER EMBRYO CELLS (DETERMINED FROM GROWTH CURVES AT DIFFERENT CELL DENSITIES AND SERUM CONCENTRATIONS) IN VARIOUS CONVENTIONAL BASAL MEDIA AND COMBINATIONS THEREOF. B. PREPARATION OF AN INTERIM MEDIUM THAT YIELDS MAXIMUM ATTACHMENT OF THESE ANCHORAGE-DEPENDENT CELLS TO PLASTIC SUBSTRATA WITHOUT LOSS OF VIABILITY AND DEVELOPMENT OF A SERUM-FREE PROLIFERATION MEDIUM THAT CAN SUPPORT THE GROWTH OF THE ATTACHED CELLS. THIS WILL BE ACHIEVED BY SELECTING THE ADDITIVES (GROWTH FACTORS, HORMONES, LIPOPROTEINS, ETC.) AFTER ASSAYING FOR THEIR DIRECT AND INDIRECT EFFECTS ON CELL GROWTH. DURING PHASE I, PROTOTYPE OF A SERUM-FREE MEDIUM WILL BE PREPARED AND PARTIALLY CHARACTERIZED WITH RESPECT TO ITS ABILITY TO SUPPORT TRANSFORMATION OF HAMSTER EMBRYO CELLS WITH 7,12-DIMETHYLBENZ(A)ANTHRACENE AS A PRELUDE TO FURTHER DEVELOPMENT. THE OBJECTIVE OF PHASE II WILL BE THE DEVELOPMENT OF MORE SPECIALIZED MEDIA FOR THIS AND OTHER CELL TYPES USED IN IN VITRO TRANSFORMATION STUDIES AND THE EVENTUAL PRODUCTION OF APPROPRIATE SERUM-FREE MEDIA ON A COMMERCIAL BASIS.

Principal Investigator:

P. thomas iype
PRINCIPAL INVESTIGATOR
3018318810

Business Contact:

Small Business Information at Submission:

Biological Research Faculty &
1007520 Tyler Place Ijamsville, MD 21754

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No