The long-term objective of this project is to establish a stable source of well-characterized antibodies to dna adducts of carcinogens/mutagens, so that these important probes can be made available for research into mechanism(s) of carcinogenesis, and for molecular cancer epidemiology to monitor… More

The objective of this project is to establish innovative screening systems that identify new chemopreventive agents, using different in vitro models of oncogenesis. three potential systems, each representing a different stage in the carcinogenic process, will be tested against selected… More

The objective of this project is to formulate defined serum-free media that can sustain the growth of primary and/or early passage rodent embryo cells that are commonly used in studies on the mechanisms of carcinogenesis as well as in the development of genetic toxicology assays. phase i studies… More

The long-term objective of this project is to establish novel recipient epithelial cell systems that will detect a wide spectrum of oncogenes through dna transfection. three types of liver cells representing different stages of the carcinogenic process will be used: a normal diploid, an immortalized… More

The long-term objectives of this project are (1) to establish a stable source of well-characterized antibodies to dna adducts of carcinogen/mutagens so that these important probes can be made available for fundamental research into the mechanism(s) of carcinogenesis, and (2) toprovide services/assay… More

The long-term objective of this project is to establish a stable source of pure endogenous hepatic proliferation inhibitor (hpi) and other related modulators of cell division. these important probes can then be made availablefor fundamental research into the regulation of cell proliferation and… More

The main objective of this phase i project was to establish a source of serum-free medium suitable for sustained growth of early passage syrian hamster embryo (she) cells. specific aims included selection of a basal medium supportive of clonal growth in limiting serum concentrations, replacement of… More

The ultimate objective of this project is to develop new methodologies to measure and distinguish between cytotoxicity and growth inhibition by toxic chemicals, carcinogenic agents and chemotherapeutic drugs. the specific aims include: (a) to establish the feasibility of measuring cell numbers in… More

The overall objective is to develop a new syrian hamster embryo (she) morphological transformation assay (mta) employing specific cell types as targets and using defined, serum-free culture media. specific aims for phase i study include: (1) isolation and characterization of specific cell types… More

The overall objective of this project is to include human prostatic cancer cell lines for discovering cancer chemotherpaeutic drugs. specific aims of phase i include establishment and characterization of new human prostatic cancer cell strains/lines suitable for drug evaluation. these studies will… More

The long-term objective of this project is to develop a laboratory test for monitoring low-level toxicity from chemical exposure at levels that do not produce acute symptoms such as cytotoxicity, but produce detectable damage later. the specific aims for the phase i investigations are to: (1)… More